可注射性自体组织工程骨的研究

　　作者：杨维东、张晓东、钱其春、唐立辉、杨连甲、毛天球

　　【摘要】目的：探索在动物体内异位构建可注射性自体组织工程骨的方法。方法：取新西兰兔髂骨骨髓基质细胞并诱导分化为成骨细胞，然后将骨髓基质成骨细胞与1.5%藻酸钠溶液混合形成骨髓基质成骨细胞/藻酸钠复合物，使细胞的终浓度为4×106/ml。以自体细胞移植方式将骨髓基质成骨细胞/藻酸钠复合物注射于新西兰兔背部皮下，用氯化钙固化为凝胶体。对标本进行X光和组织学观察. 结果：注射后4周、8周、12周在新西兰兔背部皮下形成硬结节，8周、12周X光片均显示有明显成骨现象。组织学分析显示标本有大量新骨形成，具有骨髓腔样结构。结论：应用藻酸钠复合骨髓基质成骨细胞可以通过注射方式在动物体内形成自体骨组织。

　　【关键词】组织工程 骨 藻酸盐

　　A study of injectable autogenous tissue-engineered bone
Yang Weidong, Zhang Xiaodong, Qian Qichun，et al. Stomatological College, Fourth military Medical university, Xi’an (710032)

　　【Abstract】Objective: The study investigates the utilization of carrier for delivering osteoblasts and creating autogenous bone tissue in ectopic site of animal via injection. Methods: Bone marrow cells harvested from iliac bone of New Zealand rabbits and induced to differentiate into marrow stromal osteoblasts. The osteoblasts were mixed with 1.5%alginate sodium solution to generate osteoblasts/alginate composites with final cellular density of 4×106/ml. Calcium chloride was used as cross-linking agent to gel aqueous alginate solution. The marrow stromal osteoblasts/alginate composites were injected into the dorsal subcutaneous tissue of rabbits with autogenous cells transplantation. The samples were examined with X-ray and histological analysis. Results: Four, eight and twelve weeks after injection, the hard knobbles were easily palpated under the dorsal skin of animals. On X-ray photograph the samples showed calcified image with more density than sounding soft tissue, new bone formation was observed in the osteoblasts/alginate composites in histological analysis. The osteogenesis was in association with regenerated hematopoietic bone marrow. Conclusion: These results revealed that new bone tissue could be created through the injection of alginate sodium mixed with autogenous marrow stromal osteoblasts.

　　Key words Tissue Engineering Bone Alginate

　　本研究为“973”子项目（编号：1999054308）资助课题

　　作者单位：第四军医大学口腔医学院